The My-Otome Zwei OAV scenario was based on the manga Mai-Otome by Kenetsu Satou, Masakazu Obara was the director. Masakazu Obara who had filmed My-Otome TV was invited director of My-Otome Zwei. Yuki Kajiura composed music for My-Otome Zwei OAV. Like the manga, the anime is filmed as a action.